2026-03-10T19:05:25Z https://hsuh.repo.nii.ac.jp/oai

oai:hsuh.repo.nii.ac.jp:00007433 2023-12-26T07:33:55Z 89:543

<原著>ラット耳下腺細胞のアミラーゼ分泌反応とサイクリックAMP生成に対するCa^<2+>およびEGTAの効果 <ORIGINAL ARTICLE>Effect of Ca^<2+> and EGTA on amylase secretion and cyclic AMP accumulation in rat parotid cells 相良, りか子 22365 松井, 聡子 35506 東城, 庸介 35507 松本, 仁人 35508 parotid glands amylase Ca EGTA cyclic AMP P(論文) The effects of Ca^<2+> and EGTA on rat parotid amylase secretion and cyclic AMP accumulation were investigated in vitro using a dispersed acinar cell preparation. Amylase secretion induced by isoproterenol and dibutyryl cyclic AMP was significantly enhanced by pre-treatment (30min) with more than 50μM Ca^<2+>. Depletion of intracellular Ca^<2+> by pre-treatment with 2mM EGTA reduced the amylase secretion, although the pre-treatment was unable to completely block the secretion. The pre-treatment with Ca^<2+> and EGTA did not alter the rise in cellular cyclic AMP induced by isoproterenol. These results suggest that intracellular Ca^<2+> is necessary to elicit the maximum amylase secretion and that Ca^<2+> may be involved in the exocytosis of parotid amylase at steps distal from the cyclic AMP metabolism. departmental bulletin paper 1988-12-31 application/pdf 東日本歯学雑誌 2 7 67 72 AN0008135X https://hsuh.repo.nii.ac.jp/record/7433/files/KJ00000094702.pdf https://hsuh.repo.nii.ac.jp/records/7433 jpn