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骨芽細胞及び増殖因子含有3Dバイオプリンティングスキャフォールドのin vitroおよびin vivoにおける骨再生誘導に関する研究
https://hsuh.repo.nii.ac.jp/records/2000570
https://hsuh.repo.nii.ac.jp/records/20005700ec22c1a-847e-4970-9d24-bf315b17e915
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
骨芽細胞及び増殖因子含有3Dバイオプリンティングスキャフォールドのin vitroおよびin vivoにおける骨再生誘導に関する研究 (12.6 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
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| 公開日 | 2025-11-05 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | 骨芽細胞及び増殖因子含有3Dバイオプリンティングスキャフォールドのin vitroおよびin vivoにおける骨再生誘導に関する研究 | |||||||||||||||||
| 言語 | ja | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | In vitro and in vivo evaluation of bone regeneration using 3D bioprinted scaffolds containing osteoblasts and growth factors | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | jpn | |||||||||||||||||
| 著者 |
山﨑, 祥太郎
× 山﨑, 祥太郎
× 中尾, 友也
× 飯嶋, 雅弘
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| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | 3D Bioprinting | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Scaffold | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Bone tissue engineering | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 言語 | en | |||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||
| 主題 | Bone remodeling | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| アクセス権 | ||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Autologous bone grafting is considered the gold standard for treating craniofacial bone defects due to its excellent osteoconductive and osteoinductive properties, as well as biocompatibility. However, limitations such as donor site morbidity and restricted graft availability have prompted the need for alternative bone substitutes. In particular, for orthodontic cases requiring postoperative tooth movement, conventional bone substitutes such as hydroxyapatite (HA) are inadequate, as their slow resorption impedes normal bone remodeling and may induce adverse effects such as root resorption and soft tissue ingrowth. This study aimed to develop a novel bone scaffold capable of promoting normal bone remodeling through the incorporation of osteogenic cells and growth factors. We fabricated a scaffold using a bioink composed of sodium alginate (SA) and nanocellulose fibers (NCF), The bioink incorporated osteoblasts derived from rat calvaria along with bone morphogenetic protein−2 (BMP−2) . The constructs were printed using an extrusion−based 3D bioprinter and implanted into critical−sized calvarial defects in rats. Micro−computed tomography (micro−CT) and histological analyses were performed to evaluate bone regeneration and remodeling. The scaffolds containing both osteoblasts and BMP−2 promoted robust new bone formation, characterized by thick, continuous trabecular structures connecting seamlessly with the surrounding native bone. The regenerated bone showed similar bone mineral density (BMD) and mechanical properties, including hardness and elastic modulus, compared to the host bone. Furthermore, the scaffold exhibited excellent cell viability, structural stability, and enhanced mineralization over time. These findings suggest that a 3D bioprinted scaffold incorporating osteoblasts and BMP−2 effectively induces normal bone remodeling and could serve as a promising alternative to autologous bone grafts, particularly in clinical scenarios requiring subsequent orthodontic interventions. |
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| 言語 | en | |||||||||||||||||
| bibliographic_information |
ja : 北海道医療大学歯学雑誌 en : The Dental Journal of Health Sciences University of Hokkaido 巻 44, 号 1, p. 13-22, 発行日 2025-06-30 |
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| item_10002_source_id_9 | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 18805892 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||
