@article{oai:hsuh.repo.nii.ac.jp:00007239,
 author = {松尾, 朗 and 國本, 隆明 and 矢嶋, 俊彦},
 issue = {1},
 journal = {東日本歯学雑誌},
 month = {Jun},
 note = {P(論文), The effects of diphenylhydantoin (DPH) and lipopolysaccharide (LPS) in culture medium on cell proliferation and several cell activities were investigated in human gingival fibroblasts. The cell proliferative rate was not affected by DPH ( 5μg/ml) and LPS (E. coli, 255μg/ml) in culture medium. DPH increased the total protein, hydroxyproline content and alkaline phosphatase (ALPase) activity of fibroblast cultures as compared with the control. LPS decreased the hydroxyproline content and ALPase activity and increased acid phosphatase (ACPase) activity of cultures, but no effect was seen on the total protein. On the other hand, the level of hydroxyproline content, ALPase activity and ACPase activity in DPH+LPS-cultures were also the same as those in LPS-cultures, whereas the total protein returned to the levels of DPH-cultures. The changes in weekly increase of hydroxyproline content of fibroblast cultures were associated with the changes of ALPase activity. The amount of hydroxyproline content could; be expressed almost completely by the collagen fibrogenesis index, which were obtained using a Monocellater^[○!R] that displayed the relative absorbance of cultures stained, with Van Gieson's solution. A striking difference seen between nonLPS- and LPS-cultures was the much higher level of noncollagenous protein found in the latter ones. These results indicate that the administration of LPS may stimulate the synthesis of noncollagenous protein in human gingival fibroblasts, and this was particularly enhanced in presence of DPH.},
 pages = {21--30},
 title = {ジフェニルヒダントイン(DPH)とリポポリサッカライド(LPS)のヒト歯肉由来培養線維芽細胞の細胞活性に対する影響},
 volume = {5},
 year = {1986}
}