{"created":"2023-05-15T11:31:44.046391+00:00","id":7943,"links":{},"metadata":{"_buckets":{"deposit":"7c4810ef-a3a7-4f17-8720-88b2921b74ae"},"_deposit":{"created_by":17,"id":"7943","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"7943"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00007943","sets":["89:553"]},"author_link":["23911"],"item_1693191265938":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"VoR"}]},"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1993-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"184","bibliographicPageStart":"175","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SUZUKI,Yuko/TAKEDA,Masako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"23911","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"胎生期のマウスの嗅上皮と鋤鼻器の嗅細胞の幹細胞についてブロモデオキシウリジン(BrdU)と神経細胞接着分子(NCAM)の抗体を用いた二重染色と電顕により調べた。妊娠マウスにBrdUを投与し1時間後屠殺すると,胎生12日目の嗅上皮と鋤鼻器では上皮全体にBrdU標識細胞が多数みられ,活発に分裂していることが示唆された。これらの細胞は円柱形で,少数のリボゾーム,粗面小胞体を有しNCAM陰性であった。これらの細胞の間にNCAM陽性の嗅細胞が少数みられた。胎生14日目には,円柱形細胞は上皮の基底側に限局するようになった。円柱形細胞のBrdU標識は,BrdU投与24時間後には大部分,上皮中央の嗅細胞層へ移動することから,この円柱形細胞が嗅細胞へ分化することが示唆された。胎生17～19日目には,円柱形細胞は,鋤鼻器ではNCAM陽性の丸い細胞に,嗅上皮では二種類の基底細胞(globose basal cells, basal cells proper)に置き代るようになる。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Stem cells of olfactory (receptor) cells in the olfactory epithelium and vomeronasal organ during embryonic development of mice were investigated by double immunostaining using anti-neural cell adhesion molecule (NCAM) and anti-bromodeoxyuridine (BrdU) antibodies and by electron microscopy. The columnar-shaped cells which were negative for NCAM and located throughout the epithelium on embryonic day (E) 12 and then in the basal region after E 14, were numerously labeled with BrdU, indicating active division, when BrdU was injected 1 hr before sacrifice. These cells are presumed to develop into NCAM-immunoreactive olfactory (receptor) cells of the middle region of the epithelium since numerous labelings appeared in the middle region 24 hrs after injection of BrdU. Evidence of migration of the columnar cells from olfactory epithelium along the axons was observed. On E 17, the columnar cells in the vomeronasal organ became round-shaped, located above the processes of the supporting cells, and were stained with NCAM. On the other hand, the columnar cells in the olfactory epithelium differentiated into round-shaped cells and pyramidal-shaped cells on E 19, which respectively corresponded to globose basal cells and basal cells proper seen in postnatal epithelium, although the round-shaped cells were negative for NCAM. The result suggests that the columnar cells as stem cells of olfactory (recepetor) cells observed in embryonic days differentiate into round-shaped cells in late embryonic days and that cells in the olfactory epithelium mature at a later stage than those in the VNO.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Oral Anatomy, School of Dentistry, HIGASHI-NIPPON-GAKUEN UNIVERSITY/Department of Oral Anatomy, School of Dentistry, HIGASHI-NIPPON-GAKUEN UNIVERSITY"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"東日本学園大学/東日本学園大学"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1993-12-31"}],"displaytype":"detail","filename":"KJ00000095213.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<ORIGINAL ARTICLE>Stem cells of olfactory cells during embryonic development","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/7943/files/KJ00000095213.pdf"},"version_id":"42385a8c-0d34-4f67-9ab7-0b15c46e1c94"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Bromodeoxyuridine (BrdU)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Neural cell adhesion molecule (NCAM)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Developing olfactory epithelium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Vomeronasal organ","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<ORIGINAL ARTICLE>Stem cells of olfactory cells during embryonic development","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<ORIGINAL ARTICLE>Stem cells of olfactory cells during embryonic development","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["553"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1993-12-31"},"publish_date":"1993-12-31","publish_status":"0","recid":"7943","relation_version_is_last":true,"title":["<ORIGINAL ARTICLE>Stem cells of olfactory cells during embryonic development"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-08T07:26:40.126070+00:00"}