{"created":"2023-05-15T11:31:48.517823+00:00","id":8042,"links":{},"metadata":{"_buckets":{"deposit":"41e17849-e058-4c1b-aac5-49219f51a2cb"},"_deposit":{"created_by":17,"id":"8042","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8042"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008042","sets":["89:555"]},"author_link":["24402"],"item_1693191265938":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"VoR"}]},"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-12-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"219","bibliographicPageStart":"213","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"MUTO,Toshitaka/SATO,Kenichi/KANAZAWA,Harusachi/TAKAHASHI,Kikuo/KANAZAWA,Masaaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"24402","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"骨肉腫細胞株はヒト骨肉腫治療の実験モデルになるばかりでなく,骨形成を示すため骨代謝の研究に対しても有用な実験モデルとなりうる。しかし,可移植性骨肉腫株やその細胞株は今日においても非常に少ない。われわれはすでに^<32>Pで誘発した可移植性骨肉腫を報告した。この腫瘍は継代移植においても骨形成能を維持している。今回の実験はこの骨腫瘍の細胞株を樹立すること,ならびにその生物学的特長を検索することである。その結果,1.ラット骨肉腫の細胞株(MSK,武藤-佐藤-金沢)が樹立された。2.この細胞株はin vitroにおいて強いアルカリフォスファターゼ活性とコラーゲン形成能を示した。3.MSK細胞を同系ラットの背部皮下に移植すると骨形成能を示した。4.ミリポアフィルターを用いたin VIVO骨形成実験では,フィルターの外側に骨形成が認められた。5.以上の結果より,MSK細胞は骨形成能を示すだけでなく骨誘導能の可能性が示唆された。それゆえ,この細胞株は骨芽細胞の特性の検索ばかりでなく骨誘導の研究にも有用なモデルであると考える。","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"To study the medical treatment of human osteosarcoma there is a need for experimental tumor models Moreover, osteoblastic cell line is a useful model for research of the bone and mineral metabolism. Even today, a few transplantable bone-forming osteosarcomas and their cell lines are available Previously we have reported transplantation of osteosarcoma induced by administration of ^<32>P This tumor retains the osteoid-forming ability even in serial transplantations In the present study, we established the cell line of our transplantable ratosteosarcoma and investigated the characteristics of the cell line The results were as follows 1. A cell line (MSK, Muto-Sato-Kanazawa) of rat osteosarcoma was established 2. The cell line showed intense staining of alkaline phosphatase and collagen synthesis in vitro. 3. MSK cell line also maintained the bone-forming ability when it was inoculated sub cutaneously into the back of syngenic rats 4. Osteogenesis outside a Millipore filter by MSK cells was demonstrated in vivo. 5. It was suggested that MSK cells had not only bone-forming ability but also bone-inductive ability. Therefore, MSK cell line would be a useful model for the study on the property of osteoblast and bone induction","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"The First Department of Oral Surgery, School of Dentistry, HEALTH SCIENCES UNIVERSITY OF HOKKAIDO/Department of Oral surgery, School of Medicine, CHIBA UNIVERSITY/Department of Oral surgery, School of Medicine, CHIBA UNIVERSITY/Department of Oral surgery, School of Medicine, CHIBA UNIVERSITY/The First Department of Oral Surgery, School of Dentistry, HEALTH SCIENCES UNIVERSITY OF HOKKAIDO"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部/北海道医療大学歯学部/北海道医療大学歯学部/北海道医療大学歯学部/北海道医療大学歯学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1994-12-31"}],"displaytype":"detail","filename":"KJ00000095312.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<ORIGINAL ARTICLE>Establishment and characteristics of a cell line derived from osteosarcoma induced by ^<32>P in the rat.","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8042/files/KJ00000095312.pdf"},"version_id":"05e3bd01-f032-4497-8dc5-6dd06cc7149f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cell culture","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteosarcoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblast-like cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bone-inductive ability","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cell line","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<ORIGINAL ARTICLE>Establishment and characteristics of a cell line derived from osteosarcoma induced by ^<32>P in the rat.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<ORIGINAL ARTICLE>Establishment and characteristics of a cell line derived from osteosarcoma induced by ^<32>P in the rat.","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["555"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1994-12-31"},"publish_date":"1994-12-31","publish_status":"0","recid":"8042","relation_version_is_last":true,"title":["<ORIGINAL ARTICLE>Establishment and characteristics of a cell line derived from osteosarcoma induced by ^<32>P in the rat."],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-08T07:29:48.164282+00:00"}