@article{oai:hsuh.repo.nii.ac.jp:00008319,
 author = {谷村, 明彦 and 東城, 庸介 and 松本, 仁人},
 issue = {2},
 journal = {東日本歯学雑誌},
 month = {Dec},
 note = {P(論文), Intracellular free chloride concentrations ([Cl^-]_i) in rat parotid acinar cells were determined with the Cl^- sensitive fluorescent indicator SPQ. The [Cl^-]_i was calculated from the SPQ fluorescence based on Englom's method. The resulting level of [Cl^-]_i of the rat parotid acinar cells was estimated to be 73.4±1.9mM (mean ± S.E., n=31). Stimulation with 10μM carba-chol, a muscarinic agonist, decreased the [Cl^-]_i to 50.1±3.1mM (n=12). The ED_<50> of carbachol necessary to change the [Cl^-]_i was 1.0μM. A similar decrease in [Cl^-]_i was elicited with 10nM of substance P or 10μM of phenylephrine, an α-adrenergic agonist. When the intracelular Ca^<2+> stores were previously depleted, no agonist-induced decrease in [Cl^-]_i was observed, suggesting that the decrease in [Cl^-]_i is induced by the increase in cytosolic Ca^<2+> concentration. No detectable change in [Cl^-]_i was observed when cells were stimulated with 1μM isoproterenol, a β-adrenergic agonist which induces the production of cyclic AMP. This suggests that the [Cl^-]_i in rat parotid acinar cells is not regulated by the cyclic AMP pathway.},
 pages = {271--277},
 title = {<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定},
 volume = {16},
 year = {1997}
}