{"created":"2023-05-15T11:32:01.949185+00:00","id":8319,"links":{},"metadata":{"_buckets":{"deposit":"5f1e966e-2df3-479f-940f-e8fe333d39ac"},"_deposit":{"created_by":17,"id":"8319","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8319"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008319","sets":["89:561"]},"author_link":["25288","36942","36943"],"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"277","bibliographicPageStart":"271","bibliographicVolumeNumber":"16","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"谷村, 明彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25288","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東城, 庸介","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"36942","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松本, 仁人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"36943","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Intracellular free chloride concentrations ([Cl^-]_i) in rat parotid acinar cells were determined with the Cl^- sensitive fluorescent indicator SPQ. The [Cl^-]_i was calculated from the SPQ fluorescence based on Englom's method. The resulting level of [Cl^-]_i of the rat parotid acinar cells was estimated to be 73.4±1.9mM (mean ± S.E., n=31). Stimulation with 10μM carba-chol, a muscarinic agonist, decreased the [Cl^-]_i to 50.1±3.1mM (n=12). The ED_<50> of carbachol necessary to change the [Cl^-]_i was 1.0μM. A similar decrease in [Cl^-]_i was elicited with 10nM of substance P or 10μM of phenylephrine, an α-adrenergic agonist. When the intracelular Ca^<2+> stores were previously depleted, no agonist-induced decrease in [Cl^-]_i was observed, suggesting that the decrease in [Cl^-]_i is induced by the increase in cytosolic Ca^<2+> concentration. No detectable change in [Cl^-]_i was observed when cells were stimulated with 1μM isoproterenol, a β-adrenergic agonist which induces the production of cyclic AMP. This suggests that the [Cl^-]_i in rat parotid acinar cells is not regulated by the cyclic AMP pathway.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1997-12-30"}],"displaytype":"detail","filename":"KJ00000095588.pdf","filesize":[{"value":"500.5 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8319/files/KJ00000095588.pdf"},"version_id":"8accf7f8-55ca-4a96-a7da-26ccf4796cea"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Parotid cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"SPQ","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracellular chloride","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Fluid secretion","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定","subitem_title_language":"ja"},{"subitem_title":"<ORIGINAL ARTICLE>Determination of intracellular free chloride concentrations in rat parotid acinar cells using the chloride-sensitive fluorescent indicator SPQ","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["561"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1997-12-30"},"publish_date":"1997-12-30","publish_status":"0","recid":"8319","relation_version_is_last":true,"title":["<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-28T00:42:40.238029+00:00"}