@article{oai:hsuh.repo.nii.ac.jp:00008419,
 author = {小山, 宏樹 and 中出, 修 and 斎藤, 隆史 and 賀来, 亨},
 issue = {1},
 journal = {東日本歯学雑誌},
 month = {Jun},
 note = {P(論文), The present study was undertaken to examine the effect of phenytoin on the synthesis of bone morphogenetic proteins (BMPs). The effects of phenytoin on the gene expression of BMP-1-7 in normal human mandible-derived bone cells (HOB-M cells) were evaluated by the semiquantitative reverse transcriptionpolymerase chain reaction (RT-PCR). While phenytoin increased the mRNA levels of BMP-2 in HOB-M cells, it failed to significantly affect the mRNA levels of other BMPs. The effects of phenytoin treatment on intracellular BMP-2 synthesis in HOB-M were assessed by immunocytochemistry and the effects on the secretion of BMP-2 were evaluated by dot blotting. Phenytoin significantly increased the intensity of immunocytological staining of BMP-2 and the secretion of BMP-2. Interestingly, the stimulatory effects of phenytoin on osteocalcin secretion were completely blocked by antihuman BMP-2 monoclonal antibody. In conclusion, the present study indicates that the stimulatory effects of phenytoin on osteocalcin secretion in human bone cells are, at least, in part mediated by BMP-2.},
 pages = {39--48},
 title = {正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果},
 volume = {18},
 year = {1999}
}