{"created":"2023-05-15T11:32:06.159238+00:00","id":8419,"links":{},"metadata":{"_buckets":{"deposit":"3196e164-9538-4b66-b050-fc9e666e0a01"},"_deposit":{"created_by":17,"id":"8419","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8419"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008419","sets":["89:564"]},"author_link":["25474","25475","25476","25477"],"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"48","bibliographicPageStart":"39","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小山, 宏樹","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25474","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"中出, 修","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25475","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"斎藤, 隆史","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25476","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"賀来, 亨","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25477","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The present study was undertaken to examine the effect of phenytoin on the synthesis of bone morphogenetic proteins (BMPs). The effects of phenytoin on the gene expression of BMP-1-7 in normal human mandible-derived bone cells (HOB-M cells) were evaluated by the semiquantitative reverse transcriptionpolymerase chain reaction (RT-PCR). While phenytoin increased the mRNA levels of BMP-2 in HOB-M cells, it failed to significantly affect the mRNA levels of other BMPs. The effects of phenytoin treatment on intracellular BMP-2 synthesis in HOB-M were assessed by immunocytochemistry and the effects on the secretion of BMP-2 were evaluated by dot blotting. Phenytoin significantly increased the intensity of immunocytological staining of BMP-2 and the secretion of BMP-2. Interestingly, the stimulatory effects of phenytoin on osteocalcin secretion were completely blocked by antihuman BMP-2 monoclonal antibody. In conclusion, the present study indicates that the stimulatory effects of phenytoin on osteocalcin secretion in human bone cells are, at least, in part mediated by BMP-2.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Oral Pathology, Health Sciences University of Hokkaido"},{"subitem_text_language":"en","subitem_text_value":"Department of Operative Dentistry and Endodontolgoy, School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"原著"}]},"item_2_text_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"ORIGINAL"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部口腔病理学講座"},{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部歯科保存学第二講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"1999-06-30"}],"displaytype":"detail","filename":"KJ00003417151.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8419/files/KJ00003417151.pdf"},"version_id":"6017f806-0351-4ce5-a9d2-c2623fe14f46"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"phenytoin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone morphogenetic proteins (BMPs)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"normal human bone cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osteocalcin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RT-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果","subitem_title_language":"ja"},{"subitem_title":"Effects of phenytoin on synthesis of bone morphogenetic proteins (BMPs) in normal human bone cells in vitro","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["564"],"pubdate":{"attribute_name":"PubDate","attribute_value":"1999-06-30"},"publish_date":"1999-06-30","publish_status":"0","recid":"8419","relation_version_is_last":true,"title":["正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-28T00:45:43.083846+00:00"}