@article{oai:hsuh.repo.nii.ac.jp:00008422,
 author = {室, 三之},
 issue = {1},
 journal = {東日本歯学雑誌},
 month = {Jun},
 note = {P(論文), Evidence for apoptosis in J774.1 cells by the periodontopathic bacterium Actinobacillus actinomycetemcomitans has been reported, suggesting that the ability of A. actinomycetemcomitans to promote apoptosis may be important in the initiation and development of periodontitis. This study examined the role of macrophage CD14 in the induction of apoptosis induced by A actinomycetemcomitans infection using the parent J774.1 cells and the CD14 defective mutant LR-9 cells. It was found that LR-9 cells showed a weak cytotoxic effect after being infected with A. actinomycetemcomitans Y4. Thr LR-9 cells infected with A. actinomycetemcomitans Y4 showed no increase in the population of apoptotic nuclei, compared with the non-infected cells. A smaller number of A. actinomycetemcomitans Y4 inside LR-9 cells than inside J774.1 cells was detected by confocal scanning microscopy. Futher, CD14 is a receptor for lipopolysccharide (LPS) and it has been suggested that intracellular information was transmitted in relation the LPS binding. It was examined whether stimulation of J774.1 cell with LPS prior to infection affects the subsequent apoptosis. Apoptotic cell death of LPS-stimulated J774.1 cells was found to be significantly lower than that in the controls. Significantly smaller numbers of live A. actinomycetemcomitans were recovered from the LPS-stimulated macrophages than from controls at 8 and 10th after the infection. These findings suggest that the CD14 may contribute to the phagocytosis of A. actinomycetemcomitans by J774.1 cells, and at least in part, regulate apoptotic cell death of macrophages infected with A. actinomycetemcomitans, and that the inhibitory effect of LPS on apoptosis results from an enhanced bactericidal activity.},
 pages = {87--101},
 title = {Actinobacillus actinomycetemcomitans感染マクロファージに認められるアポトーシス発現機序に関する研究},
 volume = {18},
 year = {1999}
}