@article{oai:hsuh.repo.nii.ac.jp:00008519,
 author = {根津, 顕弘 and 谷村, 明彦 and 東城, 庸介},
 issue = {1},
 journal = {東日本歯学雑誌},
 month = {Jun},
 note = {P(論文), Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^<2+>]i) were examined using the Ca^<2+> fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^<2+>]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands.},
 pages = {15--22},
 title = {<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離},
 volume = {19},
 year = {2000}
}