{"created":"2023-05-15T11:32:10.408019+00:00","id":8519,"links":{},"metadata":{"_buckets":{"deposit":"5300e159-0848-40af-8537-788d5f35dac5"},"_deposit":{"created_by":17,"id":"8519","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8519"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008519","sets":["89:566"]},"author_link":["25936","35846","35847"],"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"22","bibliographicPageStart":"15","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"根津, 顕弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"25936","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"谷村, 明彦","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"35846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"東城, 庸介","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"35847","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^<2+>]i) were examined using the Ca^<2+> fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^<2+>]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部歯科薬理学講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2000-06-30"}],"displaytype":"detail","filename":"KJ00000095790.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8519/files/KJ00000095790.pdf"},"version_id":"cd5f4c99-c52c-4156-aa87-b35b3509f882"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Submandibular gland","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ductal cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Aciner cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Intracellular Ca^<2+> concentration","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離","subitem_title_language":"ja"},{"subitem_title":"<ORIGINAL>Isolation of rat submandibular ductal cells by Percoll centrifugation","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["566"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2000-06-30"},"publish_date":"2000-06-30","publish_status":"0","recid":"8519","relation_version_is_last":true,"title":["<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-28T00:48:51.597608+00:00"}