{"created":"2023-05-15T11:32:14.503887+00:00","id":8616,"links":{},"metadata":{"_buckets":{"deposit":"3c8e154f-03fc-4d6d-9ae8-e2ed5dc36d9c"},"_deposit":{"created_by":17,"id":"8616","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8616"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008616","sets":["89:568"]},"author_link":["26099"],"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"18","bibliographicPageStart":"7","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"野中, 浩嗣","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"26099","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"This study investigated the involvement of caspase in apoptotic cell death of A. actinomycetemcomitans-infected macrophages. Two peptide inhibitors of caspases, Z-VAD-FMK and Z-DEVD-FMK, inhibited apoptotic cell death of J774.1 infected with A. actinomycetemcomitans. During the process of apoptosis, IL-1β was detected in the culture supernatants of J774.1 cells. The IL-1β secretion was blocked by the caspase-1 inhibitor, Z-VAD-FMK. Immunoblot analysis showed that the infection of A. actinomycetemcomitans to J774.1 cells induced the cleavage of Rb, suggesting that caspase-3 was activated by A. actinomycetemcomitans-infection. The cytosol from A. actinomycetemcomitans-infected J774. 1 cells induced Rb proteolysis in vitro, which was inhibited by the caspase-3 inhibitor, Z-DEVD-FMK. Furthermore, caspase-3-like activity was markedly increased in J774.1 cells infected with A. actinomycetemcomitans between 12h and 24h, which was subsequently inhibited by the addition of Z-VAD-FMK and Z-DEVD-FMK. These findings indicate that caspase-3 induces apoptosis in J774.1 cells infected with A. actinomycetemcomitans. Taken together, these results suggest that caspase-1 and caspase-3 are involved in the induction of apoptosis in A. actinomycetemcomitans-infected macrophages.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Periodontology and Endodontology School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部歯科保存学第一講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2001-06-30"}],"displaytype":"detail","filename":"KJ00000095888.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8616/files/KJ00000095888.pdf"},"version_id":"a65ff735-11d2-45f4-bbfc-8b10867be4dc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Actinobacillus actinomycetemcomitans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Macrophages","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Caspases","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与","subitem_title_language":"ja"},{"subitem_title":"<ORIGINAL REPORT>Involvement of caspases in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["568"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2001-06-30"},"publish_date":"2001-06-30","publish_status":"0","recid":"8616","relation_version_is_last":true,"title":["<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-27T23:56:31.904908+00:00"}