{"created":"2023-05-15T11:32:23.077900+00:00","id":8814,"links":{},"metadata":{"_buckets":{"deposit":"2e8b37f6-0654-467e-9ec9-5ed12f262c38"},"_deposit":{"created_by":17,"id":"8814","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"8814"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00008814","sets":["89:572"]},"author_link":["26502","37339","37340","37341","37342"],"item_1693191265938":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_type":"VoR"}]},"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"46","bibliographicPageStart":"35","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"東日本歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木村, 和代","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"26502","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣瀬, 由紀人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"37339","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"八島, 明弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"37340","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安彦, 善裕","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"37341","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"賀来, 亨","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"37342","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Pulsing electromagnetic fields (PEMF) are known to be effective in stimulation of nonunion fractures, however the mechanism of osteogenic action of PEMF has not been fully established. This study investigated the effects of PEMF on the gene expression in human osteoblastic and the human mesencymal stem cells (hMSC). The cells were exposed to the PEMF in serum-free medium and the mRNA level of the expression of extraceller matrix associated proteins were examined by quantitative RT-PCR assay using a LightCycler. For the spreading osteoblastic cells on a type I collagen surface with PEMF stimulation, there was an approximately 2 fold increase compared with the expression in the control group for BMP-2 mRNA expression. The addition of 10 ng/ml of TGF beta-1 enhanced the BMP-2 mRNA expression in the hMSC. However, PEMF exposure inhibited BMP-2 mRNA expression in hMSC under this condition. The results indicate that the mechanism of osteogenic action of PEMF may be different in different cell types or in the degree of differentiating potency. Consequently, it is implied that PEMF has the ability to regulate BMP-2 mRNA expression directly in osteogenic cells.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN0008135X","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Departments of Fixed Prosthodontics School of Dentistry, Health Sciences University of Hokkaido/Oral Pathology, School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部歯科補綴学第二講座/北海道医療大学歯学部口腔病理学講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2003-06-30"}],"displaytype":"detail","filename":"KJ00000096087.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"<原著>パルス電磁場刺激の骨芽細胞および骨芽細胞前駆細胞への影響 : 無血清培養条件下での細胞外基質タンパクmRNA発現変化","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/8814/files/KJ00000096087.pdf"},"version_id":"180d3ddd-f17f-4548-99ef-618332ab7c60"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pulsing electromagnetic fields","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoblasts","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mesencymal stem cells","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Serum-free","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"BMP-2","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<原著>パルス電磁場刺激の骨芽細胞および骨芽細胞前駆細胞への影響 : 無血清培養条件下での細胞外基質タンパクmRNA発現変化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<原著>パルス電磁場刺激の骨芽細胞および骨芽細胞前駆細胞への影響 : 無血清培養条件下での細胞外基質タンパクmRNA発現変化","subitem_title_language":"ja"},{"subitem_title":"<ORIGINAL>Effects of pulsing electromagnetic fields in osteoblasts and osteoblastic progenitor cells : Expression of extracellular protein mRNA in serum-free cultures","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["572"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2003-06-30"},"publish_date":"2003-06-30","publish_status":"0","recid":"8814","relation_version_is_last":true,"title":["<原著>パルス電磁場刺激の骨芽細胞および骨芽細胞前駆細胞への影響 : 無血清培養条件下での細胞外基質タンパクmRNA発現変化"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-28T00:02:42.553223+00:00"}