@article{oai:hsuh.repo.nii.ac.jp:00008877,
 author = {湯本, 泰弘 and 中島, 啓介 and 藤原, 純 and 池田, 雅美 and 小鷲, 悠典},
 issue = {1},
 journal = {東日本歯学雑誌},
 month = {Jun},
 note = {P(論文), Human lactoferrin (hLf) has been reported to inhibit lipopolysaccharide (LPS) interaction with CD14 by suppressing TNF-α production of LPS-stimulated monocytes. However, it is unclear whether hLf affects the chemotaxis of monocytes. The present study investigated the effects of hLf on the monocyte chemotactic response to LPS. Human monocytic cells, THP-1, were cultured in RPMI1640 medium in the presence (treated) or absence (untreated) of 1α, 25-Dihydroxyvitamin D_3 (100nM) for 24 hr.The THP-1 cells were then washed and transferred to the upper chamber set in a 24-well culture plate; the medium in the lower chamber contained either formyl-methionyl-leucyl-phenylalanine (fMLP; 100nM), LPS from Escherichia coli (10ng/ml), or purified hLf (200μg/ml). Following a 1-hr incubation, the membrane on the floor of the upper chamber was removed and stained with Diff-Quik^<[○!R]> to measure the absorbance at 595nm. Quantification of the chemotactic response of cells was made by calculation of the chemotactic index (absorbance in the presence of chemoattractants/absorbance in the absence of chemoattractants). Cell growth was monitored with Alamar Blue^<[○!R]>. The TNF-α and LDH levels in the culture supernatant were also measured. In the untreated cells, the mean chemotactic indices of fMLP, LPS, and hLf were 3.78, 3.19, and 1.75, respectively. The indices of fMLP+hLf and LPS+hLf were 3.30 and 2.09. Similar results were observed in the treated cells. Cell growth was not affected by the addition of fMLP, LPS, or hLf. The TNF-α and LDH levels in culture supernatant increased as a result of the introduction of LPS; however, the elevated levels were reduced by the addition of hLf. These findings suggest that hLf suppresxl by the neutrophils is bound to LPS, which leads to the inhibition of TNF-α release and the chemotactic response to LPS.},
 pages = {53--60},
 title = {ヒトラクフェリンがヒト単球系細胞のケモタキシスに与える影響},
 volume = {23},
 year = {2004}
}