{"created":"2023-05-15T11:32:30.426260+00:00","id":9916,"links":{},"metadata":{"_buckets":{"deposit":"f59bb114-0956-4789-905b-b07497b28729"},"_deposit":{"created_by":17,"id":"9916","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"9916"},"status":"published"},"_oai":{"id":"oai:hsuh.repo.nii.ac.jp:00009916","sets":["157:598"]},"author_link":["32105","32106","32107","32108","32109","32115","32116","32117","32118","32119"],"item_2_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-06-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"39","bibliographicPageStart":"31","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"北海道医療大学歯学雑誌","bibliographic_titleLang":"ja"}]}]},"item_2_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鳥谷, 奈保子","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"32105","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒川, 俊哉","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"32106","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"田隈, 泰信","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"32107","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安彦, 善裕","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"32108","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"溝口, 到","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"32109","nameIdentifierScheme":"WEKO"}]}]},"item_2_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"TORIYA, Naoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"32115","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ARAKAWA, Toshiya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"32116","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"TAKUMA, Taishin","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"32117","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"ABIKO, Yashuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"32118","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"MIZOGUCHI, Itaru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"32119","nameIdentifierScheme":"WEKO"}]}]},"item_2_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_2_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Versican is a large chondroitin sulphate proteoglycan distributed in a variety of interstitial connective tissue. Versican is thought to be involved in extracellular matrix assembly and various biological cellular interactions. This study examined mRNA expression of versican isoforms in the temporomandibular joint (TMJ) disc of growing rats using a real-time quantitative PCR (QPCR). Male Wistar rats of 2, 4, 8, 16 and 24 weeks of age were used. Total RNA was isolated from the TMJ discs, and cDNA was generated by reverse transcriptase (RT) reaction. The quantitative analysis of mRNA expression of the target genes, QPCR, was performed using sequence specific TaqMan probes. The copy number of versican was calculated by an external standard (plasmid including target cDNA diluted serially in log steps from 10^8 to 10 copies) and was normalized to a reference cDNA, GAPDH. The QPCR showed that expression for versican mRNA was highest at 2 weeks and decreased thereafter. With respect to mRNA expressions for each versican isoform, the mRNA expression of VO, V1, and V3 gradually decreased with the growth, while the expression of V2 increased up to 8 weeks and decreased after that. The mRNA expression of each versican isoform showed age-related changes in the TMJ discs of growing rats.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_2_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA12060694","subitem_source_identifier_type":"NCID"}]},"item_2_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Departments of Prthodontics, School of Dentistry, Health Sciences University of Hokkaido"},{"subitem_text_language":"en","subitem_text_value":"Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido"},{"subitem_text_language":"en","subitem_text_value":"Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido"},{"subitem_text_language":"en","subitem_text_value":"Department of Oral Pathology, School of Dentistry, Health Science University of Hokkaido"},{"subitem_text_language":"en","subitem_text_value":"Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido"}]},"item_2_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"原著"}]},"item_2_text_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"ORIDINAL REPORT"}]},"item_2_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部矯正歯科学講座"},{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部口腔生化学講座"},{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部口腔生化学講座"},{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部口腔病理学講座"},{"subitem_text_language":"ja","subitem_text_value":"北海道医療大学歯学部矯正歯科学講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2005-06-30"}],"displaytype":"detail","filename":"KJ00004178308.pdf","filesize":[{"value":"957.6 kB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析","objectType":"fulltext","url":"https://hsuh.repo.nii.ac.jp/record/9916/files/KJ00004178308.pdf"},"version_id":"61766dbb-87eb-4504-877a-f07885b75773"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Versican","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Ral-time quantitative PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Temporomandibular joint disc","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析","subitem_title_language":"ja"},{"subitem_title":"mRNA expression for versican isoforms in temporomandibular joint discs of growing rats : Analysis using real-time quantitative PCR with the TaqMan prove","subitem_title_language":"en"}]},"item_type_id":"2","owner":"17","path":["598"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2005-06-30"},"publish_date":"2005-06-30","publish_status":"0","recid":"9916","relation_version_is_last":true,"title":["成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-02-28T04:43:48.132085+00:00"}