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In vitro mineral induction by immobilized phosphoprotein : Effect of phosphate group on interfacial tension for mineral induction
https://hsuh.repo.nii.ac.jp/records/8415
https://hsuh.repo.nii.ac.jp/records/84154fc1e517-3e7d-4ac7-b798-e4b6a8f49777
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
In vitro mineral induction by immobilized phosphoprotein : Effect of phosphate group on interfacial tension for mineral induction (1.2 MB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1999-06-30 | |||||
| タイトル | ||||||
| タイトル | In vitro mineral induction by immobilized phosphoprotein : Effect of phosphate group on interfacial tension for mineral induction | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Phosphoprotein | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Mineral induction | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Phosphate group | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Interfacial tension | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 言語 | ja | |||||
| 記事種別(日) | ||||||
| ja | ||||||
| 原著 | ||||||
| 記事種別(英) | ||||||
| en | ||||||
| ORIGINAL | ||||||
| 著者名(英) |
ITO, Shuichi
× ITO, Shuichi× SAITO, Takashi× CRENSHAW, Miles A.× TOYOOKA, Hiroki× MATSUDA, Koichi |
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| 著者所属(英) | ||||||
| en | ||||||
| Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Dental Research Center, School of Dentistry, University of North Carolina | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Phosphoproteins are thought to play a primary role in the deposition of mineral on the collagen of dentin. We previously reported that immobilized phosphoproteins reduced activation energy for mineral induction in metastable mineralizing solutions, and that phosphate ester was essential for mineral induction. Interfacial tension is a good measure to evalute the mineral induction potential because it is a function of the activation energy. The purpose of this study was to determine the effect of phosphate ester on interfacial tension for mineral nucleation by immobilized phosphoprotein. Phosvitin from egg yolk was used as a model phophoprotein in this study. Phosvitin was cross-linked to agarose beads with divinyl sulfone. A portion of the cross-linked phosvitin was partially dephosphorylated with potato acid phosphatase. Then, samples were incubated at 37℃ in metastable solutions that do not spontaneously precipitate, and the mineral induction time was determined in the samples. The mineral formed was confirmed by X-ray diffraction to be hydroxyapatite. Using classical nucleation theory, the interfacial tension for hydroxyapatite nucleation by intact phosvitin was determined to be 91.3ergs/cm^2, it was 93.2 ergs/cm^2 for 50%-dephosphorylated phosvitin, and 98.1 ergs/cm^2 for 70%-dephosphorylated phosvitin. Mineral formation was not induced by phosvitin that had been 94%-dephosphorylated. These results indicate that the potential of hydroxyapatite nucleation of phosvitin is high as long as it has minimum number of phosphate esters for hydroxyapatite nucleation, and that the interfacial tension for hydroxyapatite nucleation by immobilized phosphoprotein is dependent on the degree of phosphorylation. | |||||
| 言語 | en | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0008135X | |||||
| 書誌情報 |
ja : 東日本歯学雑誌 巻 18, 号 1, p. 7-15, 発行日 1999-06-30 |
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