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<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与
https://hsuh.repo.nii.ac.jp/records/8616
https://hsuh.repo.nii.ac.jp/records/86167e0716a4-8eb9-4400-bee4-699ed48ca625
名前 / ファイル | ライセンス | アクション |
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<原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与 (1.4 MB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
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公開日 | 2001-06-30 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | <原著>Actinobacillus actinomycetemcomitans感染マクロファージのアポトーシス発現におけるカスパーゼの関与 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | <ORIGINAL REPORT>Involvement of caspases in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Actinobacillus actinomycetemcomitans | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Macrophages | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Caspases | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
言語 | ja | |||||
著者名(日) |
野中, 浩嗣
× 野中, 浩嗣 |
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著者所属(日) | ||||||
ja | ||||||
北海道医療大学歯学部歯科保存学第一講座 | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Periodontology and Endodontology School of Dentistry, Health Sciences University of Hokkaido | ||||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | This study investigated the involvement of caspase in apoptotic cell death of A. actinomycetemcomitans-infected macrophages. Two peptide inhibitors of caspases, Z-VAD-FMK and Z-DEVD-FMK, inhibited apoptotic cell death of J774.1 infected with A. actinomycetemcomitans. During the process of apoptosis, IL-1β was detected in the culture supernatants of J774.1 cells. The IL-1β secretion was blocked by the caspase-1 inhibitor, Z-VAD-FMK. Immunoblot analysis showed that the infection of A. actinomycetemcomitans to J774.1 cells induced the cleavage of Rb, suggesting that caspase-3 was activated by A. actinomycetemcomitans-infection. The cytosol from A. actinomycetemcomitans-infected J774. 1 cells induced Rb proteolysis in vitro, which was inhibited by the caspase-3 inhibitor, Z-DEVD-FMK. Furthermore, caspase-3-like activity was markedly increased in J774.1 cells infected with A. actinomycetemcomitans between 12h and 24h, which was subsequently inhibited by the addition of Z-VAD-FMK and Z-DEVD-FMK. These findings indicate that caspase-3 induces apoptosis in J774.1 cells infected with A. actinomycetemcomitans. Taken together, these results suggest that caspase-1 and caspase-3 are involved in the induction of apoptosis in A. actinomycetemcomitans-infected macrophages. | |||||
言語 | en | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN0008135X | |||||
書誌情報 |
ja : 東日本歯学雑誌 巻 20, 号 1, p. 7-18, 発行日 2001-06-30 |