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成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析
https://hsuh.repo.nii.ac.jp/records/9916
https://hsuh.repo.nii.ac.jp/records/9916b4960457-cb7e-4299-a142-72fb6daa5b3d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析 (957.6 kB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2005-06-30 | |||||
| タイトル | ||||||
| タイトル | 成長期ラット顎関節円板におけるversican isoform mRNAの発現 : TaqMan probeを用いたreal-time quantitative PCRによる分析 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | mRNA expression for versican isoforms in temporomandibular joint discs of growing rats : Analysis using real-time quantitative PCR with the TaqMan prove | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Versican | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Ral-time quantitative PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Temporomandibular joint disc | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 言語 | ja | |||||
| 記事種別(日) | ||||||
| ja | ||||||
| 原著 | ||||||
| 記事種別(英) | ||||||
| en | ||||||
| ORIDINAL REPORT | ||||||
| 著者名(日) |
鳥谷, 奈保子
× 鳥谷, 奈保子× 荒川, 俊哉× 田隈, 泰信× 安彦, 善裕× 溝口, 到 |
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| 著者名(英) |
TORIYA, Naoko
× TORIYA, Naoko× ARAKAWA, Toshiya× TAKUMA, Taishin× ABIKO, Yashuhiro× MIZOGUCHI, Itaru |
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| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部矯正歯科学講座 | ||||||
| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部口腔生化学講座 | ||||||
| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部口腔生化学講座 | ||||||
| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部口腔病理学講座 | ||||||
| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部矯正歯科学講座 | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Departments of Prthodontics, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Oral Pathology, School of Dentistry, Health Science University of Hokkaido | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Versican is a large chondroitin sulphate proteoglycan distributed in a variety of interstitial connective tissue. Versican is thought to be involved in extracellular matrix assembly and various biological cellular interactions. This study examined mRNA expression of versican isoforms in the temporomandibular joint (TMJ) disc of growing rats using a real-time quantitative PCR (QPCR). Male Wistar rats of 2, 4, 8, 16 and 24 weeks of age were used. Total RNA was isolated from the TMJ discs, and cDNA was generated by reverse transcriptase (RT) reaction. The quantitative analysis of mRNA expression of the target genes, QPCR, was performed using sequence specific TaqMan probes. The copy number of versican was calculated by an external standard (plasmid including target cDNA diluted serially in log steps from 10^8 to 10 copies) and was normalized to a reference cDNA, GAPDH. The QPCR showed that expression for versican mRNA was highest at 2 weeks and decreased thereafter. With respect to mRNA expressions for each versican isoform, the mRNA expression of VO, V1, and V3 gradually decreased with the growth, while the expression of V2 increased up to 8 weeks and decreased after that. The mRNA expression of each versican isoform showed age-related changes in the TMJ discs of growing rats. | |||||
| 言語 | en | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12060694 | |||||
| 書誌情報 |
ja : 北海道医療大学歯学雑誌 巻 24, 号 1, p. 31-39, 発行日 2005-06-30 |
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