| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2024-04-10 |
| タイトル |
|
|
タイトル |
ヒト歯肉線維芽細胞におけるCa2＋／陽イオン交換体遺伝子の発現解析 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Expression analysis of Ca2+/cation antiporter genes in human gingival fibroblasts |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 著者 |
蓑輪, 映里佳
金久保, 千晶
仙葉, 愼吾
Rezon, Yanuar
倉重, 圭史
齊藤, 正人
谷村, 明彦
|
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Na+/Ca2+ exchangers |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
K+−dependent Na+/Ca2+ exchangers |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Ca2+ /cation exchangers |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Human gingival fibroblast |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Phenytoin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Gingival overgrowth induced by phenytoin is thought to be associated with Ca2+ signaling. Our previous study revealed that phenytoin suppressed the Na+/Ca2+ exchanger (NCX) which was one of the Ca2+ excretion mechanisms in human gingival fibroblasts (HGF), resulting in inhibition of Ca2+ efflux and increasing intracellular Ca2+ concentration ([Ca2+]i) of HGF. However, the expression of the Ca2+/cation antiporter (CaCA) superfamily, including the NCX, in HGF is unknown. In the present study, therefore, the CaCA superfamily expressed in HGF was examined to determine the mechanism by which phenytoin increases [Ca2+]i. End−point PCR analysis showed clear expression of NCX1 in HGF, but not NCX2 or NCX3. Furthermore, weak expression of NCKX1 and NCKX3, the K+−dependent Na+/Ca2+ exchangers, and NCLX, a mitochondrial NCX, was observed. Real− time quantitative PCR results were used to further investigate the relative expression levels of these CaCAs.
The relative expression levels of NCX1, NCKX1, and NCKX3 to housekeeping gene GAPDH expression were 2% ± SE 0.5%, 0.3% ± SE 0.05%, 0.4% ± SE 0.07% and 0.1% ± SE 0.05%, respectively. From these results, the members of the CaCA superfamily present in the plasma membrane of HGF were estimated to be NCX1, NCKX1, and NCKX3, which accounted for 76.2%, 17.7%, and 6.1% of the total, respectively. The expression of NCX2 and NCX3 was not confirmed, but the expression of NCLX was confirmed. These results suggest that NCX1 is the major component of the CaCA superfamily on the plasma membrane of HGFs. The present study suggests that phenytoin primarily suppresses NCX1, leading to an increase of [Ca2+]i in HGF . NCLX, NCKX1, and NCKX3 are also thought to be suppressed by phenytoin and partially contribute to [Ca2+]i dynamics in HGF. |
|
言語 |
en |
| 書誌情報 |
ja : 北海道医療大学歯学雑誌
en : The Dental Journal of Health Sciences University of Hokkaido
巻 42,
号 2,
p. 13-20,
発行日 2023-12-31
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18805892 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ヒト歯肉線維芽細胞におけるCa2＋／陽イオン交換体遺伝子の発現解析
