| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2024-10-17 |
| タイトル |
|
|
タイトル |
Effect of Nickel and Titanium on DNA Methylation in Human Gingival fibroblast cells In Vitro |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 著者 |
Dedy, ARIWANSA
Koki, YOSHIDA
Tetsuro, MORIKAWA
Jun, SATO
Maiko, OTOMO
Masato, SAITOH
Takashi, NEZU
Yoshihiro, ABIKO
|
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Nickel |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Titanium |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
DNA demethylation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
epigenetics |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Human gingival fibroblasts |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Metallic dental materials are frequently used in clinics. Cytotoxicity and allergy tests in vitro and in vivo have primarily been used to evaluate their safety. However, whether these dental materials cause genetic modifications leading to pathological conditions in oral tissues has not been shown. Gene mutations and epigenetic alterations are the initial steps of genetic modification. Environmental factors, including chemical components and mechanical stimulations, can cause epigenetic modifications that are often involved in pathogenic changes in humans. Therefore, we hypothesize that dental materials induce specific alterations in DNA methylation. This study aims to demonstrate specific alterations in DNA methylations in human gingival fibroblast cells (HGnF) induced by metal components (Ni and Ti ) of dental materials. The mRNA expression level of IL−6 for 2 weeks was significantly higher in the HGnF cells treated with 50 μM of Ni and 10 μM of Ti compared to the controls. Quantitative methylation − specific PCR ( qMSP) revealed that the DNA methylation percentage levels of IL−6 and IFN−γ in HGnF cells treated with 10 μM of Ti for 2 weeks were significantly lower compared to the controls. In conclusion, this study found hypomethylation of IL−6 and IFN−γ, followed by their upregulated expressions in HGnF cells stimulated with Ti. Methylation levels may serve as a new cytotoxic assessment tool, even for biocompatible materials. |
|
言語 |
en |
| bibliographic_information |
ja : 北海道医療大学歯学雑誌
en : The Dental Journal of Health Sciences University of Hokkaido
巻 43,
号 1,
p. 15-21,
発行日 2024-06-30
|
| item_10002_source_id_9 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
18805892 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
Effect of Nickel and Titanium on DNA Methylation in Human Gingival fibroblast cells In Vitro (2.5 MB)
