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<原著>DMBA塗布によるハムスター頬嚢粘膜上皮の細胞増殖とBrdUの結合
https://hsuh.repo.nii.ac.jp/records/7358
https://hsuh.repo.nii.ac.jp/records/73588abd868d-719d-4479-95ab-1ef9a2e8e7a7
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
<原著>DMBA塗布によるハムスター頬嚢粘膜上皮の細胞増殖とBrdUの結合 (3.2 MB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1987-12-30 | |||||
| タイトル | ||||||
| タイトル | <原著>DMBA塗布によるハムスター頬嚢粘膜上皮の細胞増殖とBrdUの結合 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | <ORIGINAL ARTICLE>Cheek Pouch Epithelial Cell Proliferation Induced by DMBA Application and Incorporation of BrdU | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | BrdU | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | immunohistochemistry | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | monoclonal antibody | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DMBA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 言語 | ja | |||||
| 著者名(日) |
賀来, 亨
× 賀来, 亨× 中出, 修× 八重樫, 和秀× 大内, 知之× 蓑輪, 泰子× 阿部, 英二× 菅野, 秀俊× 奥山, 富三 |
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| 著者所属(日) | ||||||
| ja | ||||||
| 東日本学園大学歯学部口腔病理学講座 | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Oral Pathology, School of Dentistry, HIGASHI-NIPPON-GAKUEN UNIVERSITY | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The application of an immunohistochemical method in the detection of proliferating cells, that have incorporated thymidine analogue, 5-bromo-2-deoxyuridine (BrdU), was studied on cheek pouch mucosas of hamsters treated by three topical applications of 0.5% DMBA mineral oil. Incorporation of BrdU into newly synthesized DNA provides the basis for a simple technique to identify proliferating cells. BrdU was administered to hamsters by intraperitoneal injections at 6 hour intervals. Tissue was fixed in chilled acetone, embedded in soft paraffin, sectioned at 5μm. Deparaffinized tissue sections were incubated with an anti-BrdU antibody, and cells which had traversed the S-phase during the BrdU exposure period were identified immunohistochemically. Proliferation and migration of cheek pouch epithelial cells were identified by antibody staining after one, five, and nine exposures to BrdU. BrdU was mostly present in the nuclei of epithelial cells of the basal cell layer. The BrdU labeling Index (LI), or S-phase fraction, was determined by counting the number of BrdU-labeled cells in the tissue sections. The average LIs for non DMBA treated and DMBA-treated cheek pouch epithelia, administered a single dose of BrdU were 2.8% and 12.4% respectively. A higher proportion of labeled cells was presnt in the basal cell layer of the DMBA-treated animals than in that of the non-DMBA-treated animals. The ability to measure cell proliferation is important in the study of oral carcinogenesis. | |||||
| 言語 | en | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0008135X | |||||
| 書誌情報 |
ja : 東日本歯学雑誌 巻 6, 号 2, p. 135-142, 発行日 1987-12-30 |
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| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
