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耳下腺のアミラーゼ分泌機構 : "穴あき細胞"の利用
https://hsuh.repo.nii.ac.jp/records/7674
https://hsuh.repo.nii.ac.jp/records/767443225de6-8e4e-41b9-b230-b40adfc2ab52
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
耳下腺のアミラーゼ分泌機構 : "穴あき細胞"の利用 (1.1 MB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1991-12-30 | |||||
| タイトル | ||||||
| タイトル | 耳下腺のアミラーゼ分泌機構 : "穴あき細胞"の利用 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Permeabilized Parotid Acini : A New Approach to Amylase Exocytosis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Amylase exocytosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cAMP-dependent protein Kinase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | saponin-permeabilized oarotid acini | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 言語 | ja | |||||
| 著者名(日) |
田隈, 泰信
× 田隈, 泰信 |
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| 著者名(英) |
TAKUMA, Taishin
× TAKUMA, Taishin |
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| 著者所属(日) | ||||||
| ja | ||||||
| 東日本学園大学歯学部口腔生化学講座 | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department Oral Biochemistry, School of Dentistry, HIGASHI-NIPPON-GAKUEN UNIVERSITY | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Permeabilized cells enabled us to gain access to the intracellular milieu required for exocytosis. Various permeabilization techniques have been applied to many secretory cells including pancreatic acini, however, studies with permeabilized salivary cells were very few. Therefore, I first detailed the preparation method of saponin-permeabilized parotid acini that were highly responsive to exogenous cAMP. Saponin-permeabilized parotid acini prepared in this manner were incubated with a wide variety of cAMP analogs, including site-selective cAMP analogs and the cAMP antagonist. The results suggest that the dissociation of cAMP-dependent protein kinase into its catalytic and regulatory subunits was essential for amylase exocytosis evoked by cAMP. Nevertheless, studies using protein kinase inhibitors and protein phosphatase inhibitors have shown that cAMP-dependent protein phosphorylation mediated by the catalytic subunit was not obligatory for exocytosis. The role of the regulatory subunit in amylase exocytosis remains to be elucidated. In this article, I also review recent studies concerning the effects of Ca^<2+>, C-kinase, and GTP-binding proteins on amylase exocytosis. | |||||
| 言語 | en | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0008135X | |||||
| 書誌情報 |
ja : 東日本歯学雑誌 巻 10, 号 2, p. 63-75, 発行日 1991-12-30 |
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| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
