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<ORIGINAL>Quantification of Porphyromonas gingivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP
https://hsuh.repo.nii.ac.jp/records/8811
https://hsuh.repo.nii.ac.jp/records/88119ef582eb-f8f1-48f4-82fa-b487833bb179
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
<ORIGINAL>Quantification of Porphyromonas gingivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP (853.0 kB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||||
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| 公開日 | 2003-06-30 | |||||||
| タイトル | ||||||||
| タイトル | <ORIGINAL>Quantification of Porphyromonas gingivalis by real time PCR : new primers targeting the rgpA and rgpB gene encoding RGP | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Porphyromonas gingivalis | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Real time PCR | |||||||
| キーワード | ||||||||
| 言語 | en | |||||||
| 主題Scheme | Other | |||||||
| 主題 | Quantification | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
| 資源タイプ | departmental bulletin paper | |||||||
| ページ属性 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | P(論文) | |||||||
| 言語 | ja | |||||||
| 著者名(日) |
東日本学園大学歯学会
× 東日本学園大学歯学会
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| 著者名(英) |
KAMAGUCHI,Arihide/NAKAMURA,Reiko/OKAMOTO,Akimasa/WATANABE,Toshihiro/OHYAMA,Tohru/BABA,Hisae
× KAMAGUCHI,Arihide/NAKAMURA,Reiko/OKAMOTO,Akimasa/WATANABE,Toshihiro/OHYAMA,Tohru/BABA,Hisae
WEKO
26499
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| 著者所属(英) | ||||||||
| en | ||||||||
| Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido/Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido/Department of Oral Bacteriology, School of Dental Medicine, Turumi University/Department of Food Sciences and Technology, Faculty of Bioindustry, Tokyo University of Agriculture/Department of Food Sciences and Technology, Faculty of Bioindustry, Tokyo University of Agriculture/Deoartment of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido | ||||||||
| 抄録(英) | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | We designed new primers for the quantification of Porphyromonas gingivalis by real time PCR. The new primer set targeted the rgpA and rgpB genes that encode arginine specific cysteine proteinase (Arggingipain or Rgp), one of the putative pathogenic factors of P. gingivalis. The PCR product obtained using our primers showed no by-products by melting curve analysis. The PCR product sequence showed no significant matches to other sequences by BLAST searching of genetic databases except for matches to P. gingivalis rgpA and rgpB sequence, and could not be amplified from template derived from other oral bacteria apart from P. gingivalis. Therefore, we concluded that our primers were specific for P. gingivalis rgpA and rgpB, and could be used to quantity from 10^3 to 10^7 P. gingivalis cells when applied to real time PCR. | |||||||
| 言語 | en | |||||||
| 雑誌書誌ID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AN0008135X | |||||||
| 書誌情報 |
ja : 東日本歯学雑誌 巻 22, 号 1, p. 11-19, 発行日 2003-06-30 |
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| 出版タイプ | ||||||||
| 出版タイプ | VoR | |||||||
