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正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果
https://hsuh.repo.nii.ac.jp/records/8419
https://hsuh.repo.nii.ac.jp/records/8419fe75cc02-29f1-4570-b4d2-b4ed448757f0
名前 / ファイル | ライセンス | アクション |
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正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果 (1.2 MB)
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
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公開日 | 1999-06-30 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 正常ヒト骨芽細胞におけるBMP産生に及ぼすフェニトインの効果 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effects of phenytoin on synthesis of bone morphogenetic proteins (BMPs) in normal human bone cells in vitro | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phenytoin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | bone morphogenetic proteins (BMPs) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | normal human bone cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | osteocalcin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RT-PCR | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
言語 | ja | |||||
記事種別(日) | ||||||
ja | ||||||
原著 | ||||||
記事種別(英) | ||||||
en | ||||||
ORIGINAL | ||||||
著者名(日) |
小山, 宏樹
× 小山, 宏樹× 中出, 修× 斎藤, 隆史× 賀来, 亨 |
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著者所属(日) | ||||||
ja | ||||||
北海道医療大学歯学部口腔病理学講座 | ||||||
著者所属(日) | ||||||
ja | ||||||
北海道医療大学歯学部歯科保存学第二講座 | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Oral Pathology, Health Sciences University of Hokkaido | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Operative Dentistry and Endodontolgoy, School of Dentistry, Health Sciences University of Hokkaido | ||||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | The present study was undertaken to examine the effect of phenytoin on the synthesis of bone morphogenetic proteins (BMPs). The effects of phenytoin on the gene expression of BMP-1-7 in normal human mandible-derived bone cells (HOB-M cells) were evaluated by the semiquantitative reverse transcriptionpolymerase chain reaction (RT-PCR). While phenytoin increased the mRNA levels of BMP-2 in HOB-M cells, it failed to significantly affect the mRNA levels of other BMPs. The effects of phenytoin treatment on intracellular BMP-2 synthesis in HOB-M were assessed by immunocytochemistry and the effects on the secretion of BMP-2 were evaluated by dot blotting. Phenytoin significantly increased the intensity of immunocytological staining of BMP-2 and the secretion of BMP-2. Interestingly, the stimulatory effects of phenytoin on osteocalcin secretion were completely blocked by antihuman BMP-2 monoclonal antibody. In conclusion, the present study indicates that the stimulatory effects of phenytoin on osteocalcin secretion in human bone cells are, at least, in part mediated by BMP-2. | |||||
言語 | en | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN0008135X | |||||
書誌情報 |
ja : 東日本歯学雑誌 巻 18, 号 1, p. 39-48, 発行日 1999-06-30 |