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<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定
https://hsuh.repo.nii.ac.jp/records/8319
https://hsuh.repo.nii.ac.jp/records/8319315077c7-ebf5-43c5-a783-2c82b7128698
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
<原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定 (500.5 kB)
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| Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 1997-12-30 | |||||
| タイトル | ||||||
| タイトル | <原著>塩素感受性蛍光色素SPQによるラット耳下腺細胞内Cl-濃度の判定 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | <ORIGINAL ARTICLE>Determination of intracellular free chloride concentrations in rat parotid acinar cells using the chloride-sensitive fluorescent indicator SPQ | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Parotid cell | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | SPQ | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Intracellular chloride | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Fluid secretion | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 言語 | ja | |||||
| 著者名(日) |
谷村, 明彦
× 谷村, 明彦× 東城, 庸介× 松本, 仁人 |
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| 著者所属(日) | ||||||
| ja | ||||||
| 北海道医療大学歯学部 | ||||||
| 著者所属(英) | ||||||
| en | ||||||
| Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Intracellular free chloride concentrations ([Cl^-]_i) in rat parotid acinar cells were determined with the Cl^- sensitive fluorescent indicator SPQ. The [Cl^-]_i was calculated from the SPQ fluorescence based on Englom's method. The resulting level of [Cl^-]_i of the rat parotid acinar cells was estimated to be 73.4±1.9mM (mean ± S.E., n=31). Stimulation with 10μM carba-chol, a muscarinic agonist, decreased the [Cl^-]_i to 50.1±3.1mM (n=12). The ED_<50> of carbachol necessary to change the [Cl^-]_i was 1.0μM. A similar decrease in [Cl^-]_i was elicited with 10nM of substance P or 10μM of phenylephrine, an α-adrenergic agonist. When the intracelular Ca^<2+> stores were previously depleted, no agonist-induced decrease in [Cl^-]_i was observed, suggesting that the decrease in [Cl^-]_i is induced by the increase in cytosolic Ca^<2+> concentration. No detectable change in [Cl^-]_i was observed when cells were stimulated with 1μM isoproterenol, a β-adrenergic agonist which induces the production of cyclic AMP. This suggests that the [Cl^-]_i in rat parotid acinar cells is not regulated by the cyclic AMP pathway. | |||||
| 言語 | en | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN0008135X | |||||
| 書誌情報 |
ja : 東日本歯学雑誌 巻 16, 号 2, p. 271-277, 発行日 1997-12-30 |
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