WEKO3
アイテム
{"_buckets": {"deposit": "5300e159-0848-40af-8537-788d5f35dac5"}, "_deposit": {"created_by": 17, "id": "8519", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "8519"}, "status": "published"}, "_oai": {"id": "oai:hsuh.repo.nii.ac.jp:00008519", "sets": ["566"]}, "author_link": ["25936", "35846", "35847"], "item_2_biblio_info_14": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000-06-30", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "22", "bibliographicPageStart": "15", "bibliographicVolumeNumber": "19", "bibliographic_titles": [{"bibliographic_title": "東日本歯学雑誌", "bibliographic_titleLang": "ja"}]}]}, "item_2_creator_6": {"attribute_name": "著者名(日)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "根津, 顕弘", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "25936", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "谷村, 明彦", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "35846", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "東城, 庸介", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "35847", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_2_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^\u003c2+\u003e]i) were examined using the Ca^\u003c2+\u003e fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^\u003c2+\u003e]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands.", "subitem_description_language": "en", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN0008135X", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_language": "ja", "subitem_text_value": "北海道医療大学歯学部歯科薬理学講座"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2000-06-30"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000095790.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "\u003c原著\u003ePercoll遠心分離法によるラット顎下腺導管細胞の単離", "objectType": "fulltext", "url": "https://hsuh.repo.nii.ac.jp/record/8519/files/KJ00000095790.pdf"}, "version_id": "cd5f4c99-c52c-4156-aa87-b35b3509f882"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Submandibular gland", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ductal cell", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Aciner cell", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Intracellular Ca^\u003c2+\u003e concentration", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u003c原著\u003ePercoll遠心分離法によるラット顎下腺導管細胞の単離", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "\u003c原著\u003ePercoll遠心分離法によるラット顎下腺導管細胞の単離", "subitem_title_language": "ja"}, {"subitem_title": "\u003cORIGINAL\u003eIsolation of rat submandibular ductal cells by Percoll centrifugation", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "17", "path": ["566"], "permalink_uri": "https://hsuh.repo.nii.ac.jp/records/8519", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2000-06-30"}, "publish_date": "2000-06-30", "publish_status": "0", "recid": "8519", "relation": {}, "relation_version_is_last": true, "title": ["\u003c原著\u003ePercoll遠心分離法によるラット顎下腺導管細胞の単離"], "weko_shared_id": -1}
<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離
https://hsuh.repo.nii.ac.jp/records/8519
https://hsuh.repo.nii.ac.jp/records/85197ffc85f1-948c-4be8-905b-27e16a6fb770
名前 / ファイル | ライセンス | アクション |
---|---|---|
<原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離 (1.0 MB)
|
|
Item type | 紀要論文(ELS) / Departmental Bulletin Paper(ELS)(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2000-06-30 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | <原著>Percoll遠心分離法によるラット顎下腺導管細胞の単離 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | <ORIGINAL>Isolation of rat submandibular ductal cells by Percoll centrifugation | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Submandibular gland | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Ductal cell | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Aciner cell | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Intracellular Ca^<2+> concentration | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
言語 | ja | |||||
著者名(日) |
根津, 顕弘
× 根津, 顕弘× 谷村, 明彦× 東城, 庸介 |
|||||
著者所属(日) | ||||||
ja | ||||||
北海道医療大学歯学部歯科薬理学講座 | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido | ||||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Ductal cells were purified by Percoll centrifugation after collagenase digestion of rat submandibular glands. After centrifugation, the cells were separated into two distinct fractions. A cell fraction obtained from the interphase of the centrifuge tube contained predominantly duct-like tubular structures, while the acini sedimented to the bottom. The ductal fraction was highly enriched in kallikrein, a ductal maker. To test the functional integrity of the prepared ductal cells, the effects of the membrane receptor agonists on intracellular free calcium concentration ([Ca^<2+>]i) were examined using the Ca^<2+> fluorescent indicator fura-2. Stimulation with carbachol, phenylephrine or isoproterenol increased significantly [Ca^<2+>]i in the ductal cells. These results indicate that the Percoll centrifugation may be used to isolate ductal cellsafter digestion of submandibular glands. | |||||
言語 | en | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN0008135X | |||||
書誌情報 |
ja : 東日本歯学雑誌 巻 19, 号 1, p. 15-22, 発行日 2000-06-30 |